n-terminally binding htt antibody 2b7 ( Search Results


n-terminally binding htt antibody 2b7 (#ch03023, coriell)  (Coriell Institute for Medical Research)
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Coriell Institute for Medical Research n-terminally binding htt antibody 2b7 (#ch03023, coriell)
a Paradigm illustrating the HD patient-based disease model (fibroblasts, iPSC, cortical progenitors (25d old), and cortical neurons (35d old)) and readouts. Created with BioRender.com. b Bar plot depicting FACS quantification of NESTIN/PAX6 double-positive cells. Statistics: Welch’s test. Bars: median ± IQR. c Bar plot illustrating FACS quantification of bIII-Tubulin/CTIP2 double-positive cells. Statistics: Welch’s test. Bars: median ± IQR. d Representative pictures of cortical neurons. Scale bar 50 µm. e Illustration depicting the MSD <t>HTT</t> quantification assay, where the added protein samples bind to <t>2B7</t> antibody, used for coating the plates. The SULFO-TAG coupled antibodies D7F7 and MW1 are added for quantification of total HTT and mutant HTT, respectively. Note: numeric values from 2B7/D7F7 assay (total HTT) cannot be directly set in relation to numeric values from 2B7/MW1 assay (mutant HTT). Created with BioRender.com. f Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in fibroblasts (4 Ctrl lines, 4 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.9717); mHTT Welch’s test ( P value = 0.0737). Bars: median ± IQR. g Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in iPSC (8 Ctrl lines, 9 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.2080); mHTT Mann–Whitney test ( P value < 0.0001). Bars: median ± IQR. h Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in cortical progenitors (7 Ctrl lines, 9 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.2149); mHTT Welch’s test ( P value = 0.0061). Bars: median ± IQR. i Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in cortical neurons (7 Ctrl lines, 8 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.2781); mHTT Welch’s test ( P value = 0.0239). Bars: median ± IQR. Source data are provided as a Source Data file.
N Terminally Binding Htt Antibody 2b7 (#Ch03023, Coriell), supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a Paradigm illustrating the HD patient-based disease model (fibroblasts, iPSC, cortical progenitors (25d old), and cortical neurons (35d old)) and readouts. Created with BioRender.com. b Bar plot depicting FACS quantification of NESTIN/PAX6 double-positive cells. Statistics: Welch’s test. Bars: median ± IQR. c Bar plot illustrating FACS quantification of bIII-Tubulin/CTIP2 double-positive cells. Statistics: Welch’s test. Bars: median ± IQR. d Representative pictures of cortical neurons. Scale bar 50 µm. e Illustration depicting the MSD HTT quantification assay, where the added protein samples bind to 2B7 antibody, used for coating the plates. The SULFO-TAG coupled antibodies D7F7 and MW1 are added for quantification of total HTT and mutant HTT, respectively. Note: numeric values from 2B7/D7F7 assay (total HTT) cannot be directly set in relation to numeric values from 2B7/MW1 assay (mutant HTT). Created with BioRender.com. f Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in fibroblasts (4 Ctrl lines, 4 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.9717); mHTT Welch’s test ( P value = 0.0737). Bars: median ± IQR. g Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in iPSC (8 Ctrl lines, 9 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.2080); mHTT Mann–Whitney test ( P value < 0.0001). Bars: median ± IQR. h Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in cortical progenitors (7 Ctrl lines, 9 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.2149); mHTT Welch’s test ( P value = 0.0061). Bars: median ± IQR. i Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in cortical neurons (7 Ctrl lines, 8 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.2781); mHTT Welch’s test ( P value = 0.0239). Bars: median ± IQR. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: An alternative splicing modulator decreases mutant HTT and improves the molecular fingerprint in Huntington’s disease patient neurons

doi: 10.1038/s41467-022-34419-x

Figure Lengend Snippet: a Paradigm illustrating the HD patient-based disease model (fibroblasts, iPSC, cortical progenitors (25d old), and cortical neurons (35d old)) and readouts. Created with BioRender.com. b Bar plot depicting FACS quantification of NESTIN/PAX6 double-positive cells. Statistics: Welch’s test. Bars: median ± IQR. c Bar plot illustrating FACS quantification of bIII-Tubulin/CTIP2 double-positive cells. Statistics: Welch’s test. Bars: median ± IQR. d Representative pictures of cortical neurons. Scale bar 50 µm. e Illustration depicting the MSD HTT quantification assay, where the added protein samples bind to 2B7 antibody, used for coating the plates. The SULFO-TAG coupled antibodies D7F7 and MW1 are added for quantification of total HTT and mutant HTT, respectively. Note: numeric values from 2B7/D7F7 assay (total HTT) cannot be directly set in relation to numeric values from 2B7/MW1 assay (mutant HTT). Created with BioRender.com. f Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in fibroblasts (4 Ctrl lines, 4 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.9717); mHTT Welch’s test ( P value = 0.0737). Bars: median ± IQR. g Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in iPSC (8 Ctrl lines, 9 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.2080); mHTT Mann–Whitney test ( P value < 0.0001). Bars: median ± IQR. h Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in cortical progenitors (7 Ctrl lines, 9 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.2149); mHTT Welch’s test ( P value = 0.0061). Bars: median ± IQR. i Bar plots quantifying total (tHTT, top) and mutant (mHTT, bottom) levels in cortical neurons (7 Ctrl lines, 8 HD lines) with 2B7/D7F7 and 2B7/MW1 MSD assays, respectively. Statistics: tHTT: Welch’s test ( P value = 0.2781); mHTT Welch’s test ( P value = 0.0239). Bars: median ± IQR. Source data are provided as a Source Data file.

Article Snippet: The MSD assay plate was coated with 5ug/ml of the N-terminally binding HTT antibody 2B7 (#CH03023, Coriell) in coating buffer (15 mM Na2CO3, 35 mM NaHCO3) overnight.

Techniques: Mutagenesis, MANN-WHITNEY